Article
SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cells.
PLoS pathogens - 1 Jan 2021
Sasaki Michihito, Uemura Kentaro, Sato Akihiko, Toba Shinsuke, Sanaki Takao, Maenaka Katsumi, Hall William W, Orba Yasuko, Sawa Hirofumi
Abstract excerpt
The spike (S) protein of Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) binds to a host cell receptor which facilitates viral entry. A polybasic motif detected at the cleavage site of the S protein has been shown to broaden the cell tropism and transmissibility of the virus. Here we examine the properties of SARS-CoV-2 variants with mutations at the S protein cleavage site that undergo inefficient...
Topics
- Amino Acid Sequence
- Animals
- Caco-2 Cells
- Cell Line
- Chlorocebus aethiops
- HEK293 Cells
- Humans
- Mutation
- SARS-CoV-2
- Sequence Alignment
- Serial Passage
- Serine Endopeptidases
- Spike Glycoprotein, Coronavirus