Article
[Microassay of High-Risk Human Papillomavirus Genotype Based on R6G-ddATP/SNaPshot-Gel Fluorescence Method].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition - 1 Jan 2021
Feng Chuan-Rui, Yang Sen, Wang Yue-Qi, Liu Ying-Ke, Chen Xiu-Yuan, Zeng Shu-Shu, Luo Ya-Xiong, Gao Xiu-Feng
Abstract excerpt
OBJECTIVE: R6G-ddATP was used as a dideoxy fluorescence substrate to establish the single base end extension (SNaPShot)-gel fluorescence method for the rapid detection of the genotypes of three high-risk human papillomaviruses (HR-HPV) ( HPV18, HPV33 and HPV35) genotypes. METHODS: HPV quality control products were used as as samples, and R6G-ddATP dideoxy fluorescence reagent was used as substrate. Firstly, HPV...
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