Article
Novel multiplexed genotyping of human papillomavirus using a VeraCode-allele-specific primer extension method.
Microbiology and immunology - 1 Feb 2012
Kitamura-Muramatsu Yuri, Kusumoto-Matsuo Rika, Kondo Kazunari, Mori Seiichiro, Saito Susumu, Tsukahara Yusuke, Kukimoto Iwao
Abstract excerpt
A VeraCode-allele-specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)-DNA. Oligonucleotide primers containing HPV-type-specific L1 sequences were annealed to HPV-DNA amplified by PGMY-PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The labeled DNA was captured by VeraCode beads through hybridization, stained with a...
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