Article
FLP-Mediated Site-Specific Gene Integration in Rice.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Srivastava Vibha
Abstract excerpt
Enabling precise gene integration is important for installing traits in the plants. One of the practical methods of achieving precise gene integration is by using the yeast FLP-FRT recombination system that is efficient in directing DNA integration into the "engineered" genomic sites. The critical parameters of this method include the use of the thermostable version of FLP protein and the promoter trap design to...
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