Article
Development of enhancer trap lines for functional analysis of the rice genome.
The Plant journal : for cell and molecular biology - 1 Aug 2003
Wu Changyin, Li Xiangjun, Yuan Wenya, Chen Guoxing, Kilian Andrzej, Li Juan, Xu Caiguo, Li Xianghua, Zhou Dao-Xiu, Wang Shiping, Zhang Qifa
Abstract excerpt
Enhancer trapping has provided a powerful strategy for identifying novel genes and regulatory elements. In this study, we adopted an enhancer trap system, consisting of the GAL4/VP16-UAS elements with GUS as the reporter, to generate a trapping population of rice. Currently, 31 443 independent transformants were obtained from two cultivars using Agrobacterium-mediated T-DNA insertion. PCR tests and DNA blot...
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