Article
[Attempting to enhance the efficiency of T-DNA insertional mutant application in rice].
Yi chuan = Hereditas - 1 Jul 2007
Zhang Ya-Fang, Pan Cun-Hong, Li Ai-Hong, Tang Wen, Wu Ru, Chen Zong-Xiang, Xu Ai-Xia, Pan Xue-Biao
Abstract excerpt
T-DNA tagging method is a high throughput system for identifying and cloning novel genes from T-DNA-inserted mutant population created via genetic transformation by Agrobacterium tumefaciens. However, the efficiency of using T-DNA-inserted mutant population to clone genes in rice was much lower than in Arabidopsis. In this study, a rice tagged line with two copies of T-DNA segments inserted independently to each...
Topics
- DNA, Bacterial
- Mutagenesis, Insertional
- Oryza
- Phenotype
- Plants, Genetically Modified
- Transformation, Genetic
