Article
Genetic requirements for the targeted integration of Agrobacterium T-DNA in Saccharomyces cerevisiae.
Nucleic acids research - 1 Feb 2003
van Attikum Haico, Hooykaas Paul J J
Abstract excerpt
Agrobacterium tumefaciens delivers transferred DNA (T-DNA) into cells of plants and yeast. In plants, the T-DNA integrates at random positions into the genome by non-homologous recombination (NHR), whereas in yeast the T-DNA preferably integrates by homologous recombination (HR). Here we show that T-DNA integration by HR in yeast requires the recombination/repair proteins Rad51 and Rad52, but not Rad50, Mre11,...
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