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Preparation and culture of bone marrow-derived macrophages from mice for functional analysis

31 Dec 2020

Abstract excerpt

The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages. For...

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PMID: 33458708
DOI: 10.1016/j.xpro.2020.100246

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