Article
Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Pineda-Torra Inés, Gage Matthew, de Juan Alba, Pello Oscar M
Abstract excerpt
Macrophages are the most specialized phagocytic cells, and acquire specific phenotypes and functions in response to a variety of external triggers. Culture of bone marrow-derived or peritoneal macrophages from mice represents an exceptionally powerful technique to investigate macrophage phenotypes and functions in response to specific stimuli, resembling as much as possible the conditions observed in various...
Topics
- Animals
- Atherosclerosis
- Cell Differentiation
- Cell Separation
- Cells, Cultured
- Culture Media
- Cytokines
- Macrophages
- Macrophages, Peritoneal
- Mice
- Phenotype