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Article

An efficient culture method for generating large quantities of mature mouse splenic macrophages.

Journal of immunological methods - 30 Sept 2008

Alatery Attiya, Basta Sameh

Abstract excerpt

In this study, we established an efficient in vitro culture method for generating mature splenic macrophages (Sp-Mphi). Splenocytes were cultured in the presence of conditioned medium containing macrophage colony-stimulating factor (M-CSF) for 7 days post post-isolation and the generated Sp-Mphi were characterized phenotypically and functionally. Through this method, 9 x 10(6)/mouse Sp-Mphi were obtained in...

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Identifiers

PMID: 18675819
DOI: 10.1016/j.jim.2008.07.009

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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