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Primary mouse osteoblast and osteoclast culturing and analysis

14 Apr 2021

Abstract excerpt

Mesenchymal-derived osteoblasts play a key role in bone formation via synthesis and mineralization of the bone and bone remodeling. Osteoclasts are multinucleated cells of hematopoietic origin with a role in bone resorption. Here, we describe a protocol for generating primary cultures of these two cell types from bone tissue including the femur, tibia, and humerus of young mice. We describe methods for addressing...

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PMID: 33912848
DOI: 10.1016/j.xpro.2021.100452

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