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Validation of multiplex PCR sequencing assay of SIV

15 Jan 2021

Abstract excerpt

BACKGROUND: The generation of accurate and reproducible viral sequence data is necessary to understand the diversity present in populations of RNA viruses isolated from clinical samples. While various sequencing methods are available, they often require high quality templates and high viral titer to ensure reliable data. METHODS: We modified a multiplex PCR and sequencing approach to characterize populations of...

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PMID: 33451356
DOI: 10.1186/s12985-020-01473-0

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