Article
Generation and isolation of single cells from mouse brain with mosaic analysis with double markers-induced uniparental chromosome disomy.
STAR protocols - 18 Dec 2020
Laukoter Susanne, Amberg Nicole, Pauler Florian M, Hippenmeyer Simon
Abstract excerpt
Mosaic analysis with double markers (MADM) technology enables concomitant fluorescent cell labeling and induction of uniparental chromosome disomy (UPD) with single-cell resolution. In UPD, imprinted genes are either overexpressed 2-fold or are not expressed. Here, the MADM platform is utilized to probe imprinting phenotypes at the transcriptional level. This protocol highlights major steps for the generation and...
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