Article
Proton uptake in the reaction center mutant L210DN from Rhodobacter sphaeroides via protonated water molecules.
Biochemistry - 21 Nov 2006
Hermes Sabine, Stachnik Joanna M, Onidas Delphine, Remy André, Hofmann Eckhard, Gerwert Klaus
Abstract excerpt
The reaction center (RC) of Rhodobacter sphaeroides uses light energy to reduce and protonate a quinone molecule, QB (the secondary quinone electron acceptor), to form quinol, QBH2. Asp210 in the L-subunit has been shown to be a catalytic residue in this process. Mutation of Asp210 to Asn leads to a deceleration of reoxidation of QA- in the QA-QB --> QAQB- transition. Here we determined the structure of the...
Topics
- Electrons
- Models, Molecular
- Mutation
- Photosynthetic Reaction Center Complex Proteins
- Protein Conformation
- Protons
- Rhodobacter sphaeroides
- Spectroscopy, Fourier Transform Infrared
- Water
