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Comparison of IRES and F2A-Based Locus-Specific Multicistronic Expression in Stable Mouse Lines

21 Dec 2011

Abstract excerpt

Efficient and stoichiometric expression of genes concatenated by bi- or multi-cistronic vectors has become an invaluable tool not only in basic biology to track and visualize proteins in vivo, but also for vaccine development and in the clinics for gene therapy. To adequately compare, in vivo, the effectiveness of two of the currently popular co-expression strategies - the internal ribosome entry site (IRES)...

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PMID: 22216134
DOI: 10.1371/journal.pone.0028885

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