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Article

Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions.

PloS one - 1 Jan 2016

Li Yi, Mendiratta Saurabh, Ehrhardt Kristina, Kashyap Neha, White Michael A, Bleris Leonidas

Abstract excerpt

CRISPR/Cas9 is an enabling RNA-guided technology for genome targeting and engineering. An acute DNA binding constraint of the Cas9 protein is the Protospacer Adjacent Motif (PAM). Here we demonstrate that the PAM requirement can be exploited to specifically target single-nucleotide heterozygous mutations while exerting no aberrant effects on the wild-type alleles. Specifically, we target the heterozygous G13A...

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Source

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Identifiers

PMID: 26788852
DOI: 10.1371/journal.pone.0144970
PMCID: PMC4720446

Genes

PAM
KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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