Article
UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution.
Nature communications - 26 Nov 2020
Zurek Paul Jannis, Knyphausen Philipp, Neufeld Katharina, Pushpanath Ahir, Hollfelder Florian
Abstract excerpt
The success of protein evolution campaigns is strongly dependent on the sequence context in which mutations are introduced, stemming from pervasive non-additive interactions between a protein's amino acids ('intra-gene epistasis'). Our limited understanding of such epistasis hinders the correct prediction of the functional contributions and adaptive potential of mutations. Here we present a straightforward unique...
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