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Article

A method for expansion and retroviral transduction of mouse regulatory T cells.

Journal of immunological methods - 1 Jan 2021

Wu Dan, Wong May Q, Vent-Schmidt Jens, Boardman Dominic A, Steiner Theodore S, Levings Megan K

Abstract excerpt

Adoptive cell therapy with genetically modified regulatory T cells (Tregs) is under clinical investigation for the treatment of transplant rejection and various autoimmune conditions. A limitation of modelling this approach in mice is the lack of optimized protocols for expanding and transducing mouse Tregs. Here we describe a protocol for purifying, expanding and retrovirally transducing mouse Tregs with a...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 33221458
DOI: 10.1016/j.jim.2020.112931

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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