Article
Efficient CRISPR/Cas9 genome editing with Citrus embryogenic cell cultures.
BMC biotechnology - 10 Nov 2020
Dutt Manjul, Mou Zhonglin, Zhang Xudong, Tanwir Sameena E, Grosser Jude W
Abstract excerpt
BACKGROUND: Development of precise genome editing strategies is a prerequisite for producing edited plants that can aid in the study of gene function and help understand the genetic traits in a cultivar. Citrus embryogenic cell cultures can be used to rapidly produce a large population of genome edited transformed citrus lines. The ability to introduce specific mutations in the genome of these cells using two...
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