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Article

Structural changes induced by ligand binding drastically increase the thermostability of the Ser/Thr protein kinase TpkD from Thermus thermophilus HB8.

FEBS letters - 1 Jan 2021

Fujino Yusuke, Miyagawa Takero, Torii Masayuki, Inoue Masao, Fujii Yuki, Okanishi Hiroki, Kanai Yoshikatsu, Masui Ryoji

Abstract excerpt

Thermophilic proteins maintain their structure at high temperatures through a combination of various factors. Here, we report the ligand-induced stabilization of a thermophilic Ser/Thr protein kinase. Thermus thermophilus TpkD unfolds completely at 55 °C despite the optimum growth temperature of 75 °C. Unexpectedly, we found that the TpkD structure is drastically stabilized by its natural ligands ATP and ADP, as...

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PMID: 33159808
DOI: 10.1002/1873-3468.13996

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