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Article

The lid subdomain of DnaK is required for the stabilization of the substrate-binding site.

The Journal of biological chemistry - 7 May 2004

Moro Fernando, Fernández-Sáiz Vanesa, Muga Arturo

Abstract excerpt

We examined the effect of deletion of different segments in the helical subdomain (the so-called "lid") of the DnaK peptide-binding domain on peptide binding and protein stability. At 25 degrees C, wt DnaK and the deletion mutant proteins are able to stably bind peptides with similar affinity. Ho...

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Source

Identifiers

PMID: 14985342
DOI: 10.1074/jbc.M400921200

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-14985342-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

Related research

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Research trail→

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The lid subdomain of DnaK is required for the stabilization of the substrate-binding site.PMID 14985342Read ↗

Ionic contacts at DnaK substrate binding domain involved in the allosteric regulation of lid dynamics.Semantic proximity Read ↗

Mechanics of Hsp70 chaperones enables differential interaction with client proteins.Semantic proximity Read ↗

Monitoring conformational heterogeneity of the lid of DnaK substrate-binding domain during its chaperone cycle.Semantic proximity Read ↗

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GrpE N-terminal domain contributes to the interaction with Dnak and modulates the dynamics of the chaperone substrate binding domain.Semantic proximity Read ↗

Structural changes induced by ligand binding drastically increase the thermostability of the Ser/Thr protein kinase TpkD from Thermus thermophilus HB8.Semantic proximity Read ↗

Multistep mechanism of substrate binding determines chaperone activity of Hsp70.Semantic proximity Read ↗

Solution conformation of wild-type E. coli Hsp70 (DnaK) chaperone complexed with ADP and substrate.Semantic proximity Read ↗

A GrpE mutant containing the NH(2)-terminal "tail" region is able to displace bound polypeptide substrate from DnaK.Semantic proximity Read ↗

Thermodynamic dissection of large-scale domain motions coupled with ligand binding of enzyme I.Semantic proximity Read ↗

Conserved Helix-Flanking Prolines Modulate Intrinsically Disordered Protein:Target Affinity by Altering the Lifetime of the Bound Complex.Semantic proximity Read ↗

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