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Article

Protocol for Genome Editing to Produce Multiple Mutants in Wheat.

STAR protocols - 18 Sept 2020

Abe Fumitaka, Ishida Yuji, Hisano Hiroshi, Endo Masaki, Komari Toshihiko, Toki Seiichi, Sato Kazuhiro

Abstract excerpt

Here, we describe a protocol for producing multiple recessive mutants via genome editing in hexaploid wheat (Triticum aestivum) cv. Fielder. Using Agrobacterium-delivered CRISPR/Cas9 and three sub-genome-specific primer sets, all possible combinations of single, double, and triple transgene-free mutants can be generated. The technique for acceleration of generation advancement with embryo culture reduces time for...

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Identifiers

PMID: 33111098
DOI: 10.1016/j.xpro.2020.100053
PMCID: PMC7580089

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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