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In-planta Agrobacterium-mediated cereal transformation-InPACT.

Plant physiology - 2 Apr 2026

Nepal Nirman, Rafiei Fariba, Scofield Steve, Zhang Cankui, Mohammadi Mohsen

Abstract excerpt

We present a simple, efficient, and genotype-flexible method for transformation of small grain cereals. The method is named In-planta Agrobacterium-mediated cereal transformation, or InPACT. We demonstrate the genotype flexibility of InPACT through successful transformation of 32 different wheat genotypes: 29 hexaploid (Triticum aestivum) and 3 tetraploid durum (Triticum durum) wheat accessions showing global...

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PMID: 41741944
DOI: 10.1093/plphys/kiag069

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