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Genome Editing to Produce Knockout Mutations of Seed Dormancy Genes in Wheat.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024

Abe Fumitaka, Kamiya Yoko, Ishida Yuji, Hisano Hiroshi, Kawaura Kanako, Komari Toshihiko, Sato Kazuhiro

Abstract excerpt

Knockout mutants provide definitive information about the functions of genes related to agronomic traits, including seed dormancy. However, it takes many years to produce knockout mutants using conventional techniques in polyploid plants such as hexaploid wheat. Genome editing with sequence-specific nucleases is a promising approach for obtaining knockout mutations in all targeted homoeologs of wheat...

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PMID: 38977575
DOI: 10.1007/978-1-0716-3965-8_13

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