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Identification of a Polymorphism in the N Gene of SARS-CoV-2 That Adversely Impacts Detection by Reverse Transcription-PCR

19 Oct 2020

Abstract excerpt

Since 7 April 2020, our coronavirus disease 2019 (COVID-19) diagnostic laboratory (CLIAHUB) has received samples from multiple counties in California. Our reverse transcriptase PCR (RT-PCR) protocol (1) employs N-gene (NIID_2019-nCov_N_F2/R2ver3/P2 [Japan]) (2) and E-gene (E_Sarbeco_F/R/P1 [Germany]) (3) simplex assays. In July 2020, we identified 40+ samples from Madera County with poor N-gene assay performance...

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PMID: 33067272
DOI: 10.1128/jcm.02369-20

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