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Article

Rapid establishment of human colonic organoid knockout lines

12 Apr 2022

Abstract excerpt

Human colonic organoids derived from biopsy or autopsy tissues are a vital tool to study mucosal homeostasis, model colonic diseases, and develop therapeutics. Rapid and reliable generation of knockout organoid lines from multiple donors enables analysis of specific gene functions. Here, we report protocols to produce colonic organoid knockout lines within 1 to 2 weeks using lentiviral delivery of CRISPR-Cas9,...

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PMID: 35463469
DOI: 10.1016/j.xpro.2022.101308

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