Article
Rapid detection of rifampicin-resistant Mycobacterium tuberculosis, based on isothermal DNA amplification and DNA chromatography.
Journal of microbiological methods - 1 Oct 2020
Takarada Yutaka, Kodera Takuya, Kobayashi Kumi, Nakajima Chie, Kawase Mitsuo, Suzuki Yasuhiko
Abstract excerpt
Rapid and easy detection of nucleotide point mutations in bacterial pathogens associated with drug resistance is essential for the proper use of antimicrobials. Here, we developed a rapid and simple method for the detection of mutations using Loop-mediated isothermal amplification (LAMP) combined with the single-tag hybridization (STH) chromatographic printed array strips (PAS) method. This procedure is able to...
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