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Article

Escherichia coli flagellar genes as target sites for integration and expression of genetic circuits.

PloS one - 1 Jan 2014

Juhas Mario, Evans Lewis D B, Frost Joe, Davenport Peter W, Yarkoni Orr, Fraser Gillian M, Ajioka James W

Abstract excerpt

E. coli is a model platform for engineering microbes, so genetic circuit design and analysis will be greatly facilitated by simple and effective approaches to introduce genetic constructs into the E. coli chromosome at well-characterised loci. We combined the Red recombinase system of bacteriophage λ and Isothermal Gibson Assembly for rapid integration of novel DNA constructs into the E. coli chromosome. We...

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Identifiers

PMID: 25350000
DOI: 10.1371/journal.pone.0111451
PMCID: PMC4211737

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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