Genetic manipulation of the obligate chemolithoautotrophic bacterium Thiobacillus denitrificans.

Abstract excerpt

Chemolithoautotrophic bacteria can be of industrial and environmental importance, but they present a challenge for systems biology studies, as their central metabolism deviates from that of model organisms and there is a much less extensive experimental basis for their gene annotation than for typical organoheterotrophs. For microbes with sequenced genomes but unconventional metabolism, the ability to create...

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