Article
Targeted Amplicon Sequencing for Single-Nucleotide-Polymorphism Genotyping of Attaching and Effacing Escherichia coli O26:H11 Cattle Strains via a High-Throughput Library Preparation Technique.
Applied and environmental microbiology - 15 Jan 2016
Ison Sarah A, Delannoy Sabine, Bugarel Marie, Nagaraja Tiruvoor G, Renter David G, den Bakker Henk C, Nightingale Kendra K, Fach Patrick, Loneragan Guy H
Abstract excerpt
Enterohemorrhagic Escherichia coli (EHEC) O26:H11, a serotype within Shiga toxin-producing E. coli (STEC) that causes severe human disease, has been considered to have evolved from attaching and effacing E. coli (AEEC) O26:H11 through the acquisition of a Shiga toxin-encoding gene. Targeted amplicon sequencing using next-generation sequencing technology of 48 phylogenetically informative single-nucleotide...
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