Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A novel method for isolating lymphatic endothelial cells from lymphatic malformations and detecting PIK3CA somatic mutation in these isolated cells.

Surgery today - 1 Mar 2021

Usui Hidehito, Tsurusaki Yoshinori, Shimbo Hiroko, Saitsu Hirotomo, Harada Noriaki, Kitagawa Norihiko, Mochizuki Kyoko, Masuda Munetaka, Kurosawa Kenji, Shinkai Masato

Abstract excerpt

PURPOSE: Tissue disaggregation and the cell sorting technique by surface markers has played an important role in isolating lymphatic endothelial cells (LECs) from lymphatic malformation (LM). However, this technique may have the drawback of impurities or result in isolation failure because it is dependent on surface marker expressions, the heterogeneity of which has been found in the lymphatic system. We...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Separation
Share to Child

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Class I Phosphatidylinositol 3-Kinases

Share to Endothelial Cells

Share to Gene Expression

Share to Genetic Heterogeneity

Share to Infant

Share to Lymphatic System

Source

Identifiers

PMID: 32876734
DOI: 10.1007/s00595-020-02122-3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A novel method for isolating lymphatic endothelial cells from lymphatic malformations and detecting PIK3CA somatic mutation in these isolated cells.PMID 32876734Read ↗

PIK3CA mutations are specifically localized to lymphatic endothelial cells of lymphatic malformations.Semantic proximity Read ↗

Constitutively active PIK3CA mutations are expressed by lymphatic and vascular endothelial cells in capillary lymphatic venous malformation.Semantic proximity Read ↗

AKT hyper-phosphorylation associated with PI3K mutations in lymphatic endothelial cells from a patient with lymphatic malformation.Semantic proximity Read ↗

Activating PIK3CA alleles and lymphangiogenic phenotype of lymphatic endothelial cells isolated from lymphatic malformations.Semantic proximity Read ↗

Single-Cell RNA Sequencing Reveals Heterogeneity and Functional Diversity of Lymphatic Endothelial CellsSemantic proximity Read ↗

Immunoregulatory subtype of dermal lymphatic endothelial cells at capillary terminals drives lymphatic malformationsSemantic proximity Read ↗

Isolation, purification, and heterogeneity of human lymphatic endothelial cells from different tissues.Semantic proximity Read ↗

A somatic mutation in PIK3CD unravels a novel candidate gene for lymphatic malformation.Semantic proximity Read ↗

Molecules That Have Rarely Been Studied in Lymphatic Endothelial CellsSemantic proximity Read ↗

Feeder-Free Generation of Lymphatic Endothelial Cells from Human Induced Pluripotent Stem CellsSemantic proximity Read ↗

MEK / ERK inhibitor effectively impact generalized lymphatic anomaly (GLA) cells growth through EGFR / MEK /ERK signaling pathwaySemantic proximity Read ↗

Non-hotspot PIK3CA mutations are more frequent in CLOVES than in common or combined lymphatic malformations.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.