Article
Methods to Map Meiotic Recombination Proteins in Saccharomyces cerevisiae.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Sanchez Aurore, Borde Valérie
Abstract excerpt
Meiotic recombination is triggered by programmed DNA double-strand breaks (DSBs), catalyzed by the type II topoisomerase-like Spo11 protein. Meiotic DSBs are repaired by homologous recombination, which produces either crossovers or noncrossovers, this decision being linked to the binding of proteins specific of each pathway. Mapping the binding of these proteins along chromosomes in wild type or mutant yeast...
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