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Article

Improving the diversity of captured full-length isoforms using a normalized single-molecule RNA-sequencing method.

Communications biology - 30 Jul 2020

Hu Yueming, Shu Xing-Sheng, Yu Jiaxian, Sun Ming-An, Chen Zewei, Liu Xianming, Fang Qiongfang, Zhang Wei, Hui Xinjie, Ying Ying, Fu Li, Lu Desheng, Kumar Rakesh, Wang Yejun

Abstract excerpt

Human genes form a large variety of isoforms after transcription, encoding distinct transcripts to exert different functions. Single-molecule RNA sequencing facilitates accurate identification of the isoforms by extending nucleotide read length significantly. However, the gene or isoform diversity is lowly represented by the mRNA molecules captured by single-molecule RNA sequencing. Here, we show that a cDNA...

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Identifiers

PMID: 32732980
DOI: 10.1038/s42003-020-01125-7
PMCID: PMC7393167

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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