Article
Comparison of the Clinical Validity of Droplet Digital PCR to ARMS-PCR for BRAF V600E Mutation Detection in Thyroid Nodules.
Journal of clinical laboratory analysis - 1 Nov 2020
Li Xiubo, Du Hong, Luo Jingyan, Ding Wenshuang, Lai Bingquan, He Jiqian, Xu Shaofei, Zhang Yuying
Abstract excerpt
OBJECTIVES: Droplet digital PCR (ddPCR) has been reported to have a superior validity over PCR with amplification-refractory mutation system (ARMS-PCR) for detecting the BRAF V600E mutation in thyroid nodule fine-needle aspiration (FNA) samples using cytological diagnosis as the reference. Howeve...
Topics
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Biopsy, Fine-Needle
- DNA Mutational Analysis
- Female
- Humans
- Male
- Middle Aged
- Mutation
- Polymerase Chain Reaction
- Predictive Value of Tests
- Proto-Oncogene Proteins B-raf
- Reproducibility of Results