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Evaluation of variant calling for cpn60 barcode sequence-based microbiome profiling

9 Jul 2020

Abstract excerpt

Amplification and sequencing of conserved genetic barcodes such as the cpn60 gene is a common approach to determining the taxonomic composition of microbiomes. Exact sequence variant calling has been proposed as an alternative to previously established methods for aggregation of sequence reads into operational taxonomic units (OTU). We investigated the utility of variant calling for cpn60 barcode sequences and...

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PMID: 32645030
DOI: 10.1371/journal.pone.0235682

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