Article
Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutational processes.
Nature communications - 5 Jul 2024
Jia Hangxing, Tan Shengjun, Cai Yingao, Guo Yanyan, Shen Jieyu, Zhang Yaqiong, Ma Huijing, Zhang Qingzhu, Chen Jinfeng, Qiao Gexia, Ruan Jue, Zhang Yong E
Abstract excerpt
Long-read sequencing, exemplified by PacBio, revolutionizes genomics, overcoming challenges like repetitive sequences. However, the high DNA requirement ( > 1 µg) is prohibitive for small organisms. We develop a low-input (100 ng), low-cost, and amplification-free library-generation method for PacBio sequencing (LILAP) using Tn5-based tagmentation and DNA circularization within one tube. We test LILAP with two...
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