Article
ULK complex organization in autophagy by a C-shaped FIP200 N-terminal domain dimer.
The Journal of cell biology - 6 Jul 2020
Shi Xiaoshan, Yokom Adam L, Wang Chunxin, Young Lindsey N, Youle Richard J, Hurley James H
Abstract excerpt
The autophagy-initiating human ULK complex consists of the kinase ULK1/2, FIP200, ATG13, and ATG101. Hydrogen-deuterium exchange mass spectrometry was used to map their mutual interactions. The N-terminal 640 residues (NTD) of FIP200 interact with the C-terminal IDR of ATG13. Mutations in these regions abolish their interaction. Negative stain EM and multiangle light scattering showed that FIP200 is a dimer,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.