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Monitoring of tisagenlecleucel transgene DNA using a quantitative polymerase chain reaction assay

10 Dec 2020

Abstract excerpt

Chimeric antigen receptor (CAR)-T cell therapies reprogram T cells to engage and eliminate cancer cells. Patients' T cells are transduced in vitro using lentiviral or retroviral vectors containing a CAR transgene. Following infusion, CAR-T cells expand in vivo and may persist in the peripheral blood and bone marrow for years. Therefore, monitoring in vivo copies of the CAR transgene requires highly sensitive,...

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PMID: 33614827
DOI: 10.1016/j.omtm.2020.12.002

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