Layered engineering of sucrose phosphorylase to control substrate tunnels for efficient industrial bioproduction of 2‑O‑α‑D‑glucopyranosyl‑L‑ascorbic acid.

Abstract excerpt

The scale-up industrial production of functional glucosides via enzyme-driven biocatalysis continues to face several critical challenges, including the scarcity of robust enzymes, unclear activity regulation mechanisms, and suboptimal compatibility with industrial requirements. Here, a layered mutation strategy was employed to rationally engineer the substrate tunnel of sucrose phosphorylase (SPase), achieving...

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• Ascorbic Acid
• Bioreactors
• Glucosyltransferases
• Mutation
• Protein Engineering