Article
A Synthetic Genetic Circuit Enables Precise Quantification of Direct Repeat Deletion in Bacteria.
ACS synthetic biology - 15 May 2020
Huang Yajia, Yang Shuai, Chen Wenhui, Li Feixuan, Xia Aiguo, Ni Lei, Yang Guang, Jin Fan
Abstract excerpt
Quantification of the rate of direct repeat deletion (DRD) is an important aspect in the research of DNA rearrangement. The widely used tetracycline selection method usually introduces antibiotic pressure to the tested organism, which may interfere with the DRD process. Also the length of repeat arm (LRA) is limited by the length of the TetR coding sequence. On the basis of the fluorescent microscopy and...
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