Article
CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities.
Nature - 1 Apr 2020
Han Kyuho, Pierce Sarah E, Li Amy, Spees Kaitlyn, Anderson Gray R, Seoane Jose A, Lo Yuan-Hung, Dubreuil Michael, Olivas Micah, Kamber Roarke A, Wainberg Michael, Kostyrko Kaja, Kelly Marcus R, Yousefi Maryam, Simpkins Scott W, Yao David, Lee Keonil, Kuo Calvin J, Jackson Peter K, Sweet-Cordero Alejandro, Kundaje Anshul, Gentles Andrew J, Curtis Christina, Winslow Monte M, Bassik Michael C
Abstract excerpt
Cancer genomics studies have identified thousands of putative cancer driver genes1. Development of high-throughput and accurate models to define the functions of these genes is a major challenge. Here we devised a scalable cancer-spheroid model and performed genome-wide CRISPR screens in 2D monolayers and 3D lung-cancer spheroids. CRISPR phenotypes in 3D more accurately recapitulated those of in vivo tumours, and...
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