Article
Comparison of sequencing methods and data processing pipelines for whole genome sequencing and minority single nucleotide variant (mSNV) analysis during an influenza A/H5N8 outbreak.
PloS one - 1 Jan 2020
Poen Marjolein J, Pohlmann Anne, Amid Clara, Bestebroer Theo M, Brookes Sharon M, Brown Ian H, Everett Helen, Schapendonk Claudia M E, Scheuer Rachel D, Smits Saskia L, Beer Martin, Fouchier Ron A M, Ellis Richard J
Abstract excerpt
As high-throughput sequencing technologies are becoming more widely adopted for analysing pathogens in disease outbreaks there needs to be assurance that the different sequencing technologies and approaches to data analysis will yield reliable and comparable results. Conversely, understanding where agreement cannot be achieved provides insight into the limitations of these approaches and also allows efforts to be...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.