Article
Shall I trust the report? Variable performance of Sanger sequencing revealed by deep sequencing on HIV drug resistance mutation detection.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - 1 Apr 2020
Chen Nan-Yu, Kao Shu-Wei, Liu Zhuo-Hao, Wu Ting-Shu, Tsai Chia-Lung, Lin Hsi-Hsun, Wong Wing-Wai, Chang Yea-Yuan, Chen Shu-Sheng, Ku Stephane Wen-Wei
Abstract excerpt
BACKGROUND: The clinical utilisation of deep sequencing in HIV treatment has been hindered due to its unknown correlation with standard Sanger genotyping and the undetermined value of minority drug resistance mutation (DRM) detection. OBJECTIVES: To compare deep sequencing performance to standard Sanger genotyping with clinical samples, in an effort to delineate the correlation between the results from the two...
Topics
- Adult
- Anti-HIV Agents
- Drug Resistance, Viral
- Genotyping Techniques
- HIV Infections
- HIV-1
- High-Throughput Nucleotide Sequencing
- Humans
- Male
- Middle Aged
- Mutation
- Retrospective Studies