Article
Characterization of a TP53 Somatic Variant of Unknown Function From an Ovarian Cancer Patient Using Organoid Culture and Computational Modeling.
Clinical obstetrics and gynecology - 1 Mar 2020
Bi Jianling, Thiel Kristina W, Litman Jacob M, Zhang Yuping, Devor Eric J, Newtson Andreea M, Schnieders Michael J, Gonzalez Bosquet Jesus, Leslie Kimberly K
Abstract excerpt
In our proof-of-concept study of 1 patient with stage IIIC carcinosarcoma of the ovary, we discovered a rare mutation in the tumor suppressor, TP53, that results in the deletion of N131. Immunofluorescence imaging of the organoid culture revealed hyperstaining of p53 protein. Computational modeling suggests this residue is important for maintaining protein conformation. Drug screening identified the combination...
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