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Article

One-step triplex high-resolution melting analysis for rapid identification and simultaneous subtyping of frequently isolated Salmonella serovars.

Applied and environmental microbiology - 1 May 2012

Zeinzinger Josef, Pietzka Ariane T, Stöger Anna, Kornschober Christian, Kunert Renate, Allerberger Franz, Mach Robert, Ruppitsch Werner

Abstract excerpt

Salmonellosis is one of the most important food-borne diseases worldwide. For outbreak investigation and infection control, accurate and fast subtyping methods are essential. A triplex gene-scanning assay was developed and evaluated for serotype-specific subtyping of Salmonella enterica isolates based on specific single-nucleotide polymorphisms in fragments of fljB, gyrB, and ycfQ. Simultaneous gene scanning of...

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Identifiers

PMID: 22344662
DOI: 10.1128/AEM.07668-11
PMCID: PMC3346493

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