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Article

Functional characterization of MCAK/Kif2C cancer mutations using high-throughput microscopic analysis.

Molecular biology of the cell - 19 Mar 2020

Wagenbach Mike, Vicente Juan Jesus, Ovechkina Yulia, Domnitz Sarah, Wordeman Linda

Abstract excerpt

The microtubule (MT)-depolymerizing activity of MCAK/Kif2C can be quantified by expressing the motor in cultured cells and measuring tubulin fluorescence levels after enough hours have passed to allow tubulin autoregulation to proceed. This method allows us to score the impact of point mutations within the motor domain. We found that, despite their distinctly different activities, many mutations that impact...

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Identifiers

PMID: 31746663
DOI: 10.1091/mbc.E19-09-0503
PMCID: PMC7202071

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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