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Article

Pathogen identification by shotgun metagenomics of patients with necrotizing soft‐tissue infections

14 Oct 2019

Abstract excerpt

BACKGROUND: Necrotizing soft-tissue infections (NSTIs) are life threatening, requiring broad-spectrum antibiotics. Their aetiological diagnosis can be limited by poor performance of cultures and administration of antibiotics before surgery. OBJECTIVES: We aimed (i) to compare 16S-targeted metagenomics (TM) and unbiased semiquantitative panmicroorganism DNA- and RNA-based shotgun metagenomics (SM) with cultures,...

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PMID: 31610037
DOI: 10.1111/bjd.18611

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