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Article

Efficient RNP-directed Human Gene Targeting Reveals SPDEF Is Required for IL-13–induced Mucostasis

9 Oct 2019

Abstract excerpt

Abstract Primary human bronchial epithelial cell (HBEC) cultures are a useful model for studies of lung health and major airway diseases. However, mechanistic studies have been limited by our ability to selectively disrupt specific genes in these cells. Here we optimize methods for gene targeting in HBECs by direct delivery of single guide RNA (sgRNA) and rCas9 (recombinant Cas9) complexes by electroporation,...

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Identifiers

PMID: 31596609
DOI: 10.1165/rcmb.2019-0266oc

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