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Real-time PCR and targeted next-generation sequencing in the detection of low level EGFR mutations: Instructive case analyses

1 Jan 2019

Abstract excerpt

Allele specific real-time PCR and next-generation sequencing (NGS) are widely used to detect somatic mutation in non-small cell lung cancer (NSCLC). Both methods commonly use formalin-fixed paraffin-embedded (FFPE) tissues as diagnostic materials. Real-time PCR has the advantage of being easy to use and more tolerant of variable DNA quality, but has limited multiplex capability. NGS, in contrast, allows...

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PMID: 31367517
DOI: 10.1016/j.rmcr.2019.100901

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