Article
Frequent functional activation of RAS signalling not explained by RAS/RAF mutations in relapsed/refractory multiple myeloma.
Scientific reports - 10 Sept 2018
Wong Kwan Yeung, Yao Qiumei, Yuan Ling-Qing, Li Zhenhai, Ma Edmond Shiu Kwan, Chim Chor Sang
Abstract excerpt
RAS mutations are frequent in relapsed/refractory multiple myeloma (RRMM) but functional study in primary samples is scanty. Herein, in primary myeloma plasma cells of 17 suspected RRMM, functional activation of RAS signalling was studied by Western blot of phosphorylated ERK1/2 (phospho-ERK1/2). Moreover, activating mutations in KRAS, NRAS, BRAF, and ALK were studied by PCR and bidirectional direct sequencing....
Topics
- Cell Line, Tumor
- DNA Methylation
- DNA Mutational Analysis
- Drug Resistance, Neoplasm
- Female
- Humans
- MAP Kinase Signaling System
- Multiple Myeloma
- Mutation
- Neoplasm Recurrence, Local
- Primary Cell Culture
- Protein Kinase Inhibitors
- raf Kinases
- ras Proteins